Nuclear Magnetic Resonance
nuclear magnetic resonance
Shared Resource Director: Carol B. Post
Facility Director: John Harwood
Department: Chemistry/Medicinal Chemistry
Phone: (765) 494-5287
Building/Room: WTHR, Room 365B
General Information and Services
The Nuclear Magnetic Resonance (NMR) Shared Resource provides a broad spectrum of NMR instrumentation and scientific expertise for cancer research. The Resource maintains medium and high-field spectrometers housed across campus for easy access by all Center for Cancer Research researchers.
NMR spectroscopy plays a central role across many areas of cancer research. The Resource serves research projects in structural biology as well as synthesis and analysis of complex natural products and synthetic molecules.
The Facility provides training and access to a number of NMR spectrometers, ranging from walk-on use of 200-, 300-, and 500-MHz multinuclear spectrometers to weeklong use of 500- and 600-MHz multinuclear spectrometers with gradient capabilities.
Researchers are taught to operate the instruments by expert staff members, and data can be processed either by off-line computers available in the resource or by electronic transfer to user-owned computers.
Two Ph.D. spectroscopists, an instrumentation engineer, and an instrumentation specialist staff the NMR Resource. Staff members are responsible for maintaining the instruments, scheduling, training, and day-to-day operations.
NMR spectroscopy plays a critical role in a variety of cancer research projects. Studies to determine 3-dimensional structure and elucidate protein-protein interactions are underway for several signaling proteins including Src-family tyrosine kinases, tyrosine phosphatases, Syk and a tumor suppressor protein. These studies demand high-field multiresonance NMR spectroscopy with highest sensitivity and resolution.
Structure elucidation of proteins associated with abnormal cell growth add to our knowledge of cell growth regulation and enhance the development of agents with potential therapeutic use.
Current research that relies on NMR spectroscopy also includes the development of synthetic protocols for natural products, such as cephalostatin, and other small-molecule inhibitors with chemotherapeutic activity. Further studies are ongoing to design and synthesize new phosphoramidate anticancer prodrugs. NMR is used routinely to characterize synthetic intermediates and follow activation kinetics.