Flow Cytometry

 



Flow Cytometry

Shared Resource Director:
James Leary
E-mail: jfleary@purdue.edu
Shared Resource Manager: Jeffrey Woodliff
Email:jwoodlif@purdue.edu
Phone: (765) 494 – 3505, (414) 943 - 1113
Building/Room: BIND 233
 
The Purdue Flow Cytometry and Cell Separation Shared Resource (FC-SR) provides advanced cell and particle analysis, and sorting capabilities to Purdue University Center for Cancer Research (PCCR) members.  As an analytical tool, flow cytometry allows users to identify and quantitate cellular features, organelles, or structural components using optical measures.  When combined with sorting capabilities, it is possible to identify and isolate single cells that have specific properties or express specific molecules and which can then be further examined and characterized.  In cancer research, where the identification and characterization of unique, specific cells with cancer-like characteristics is critical, flow cytometry and cell sorting are extremely vital and useful to today’s cancer researcher.
 
The FC-SR, located in the Bindley Bioscience Center (BBC) of Discovery Park, has grown considerably in both its technical expertise and instrument capabilities since its total re-organization in March 2009.  A major emphasis of the facility is live cell sorting of mouse and human cells under BSL-2 conditions. In 2010 with support from an NIH S10 Shared Instrument Grant, the FC-SR obtained a high-throughput LEAP image scanning cytometer. This instrument adds an additional dimension to sorting of attached cells by laser ablation and serves as a bridge between flow cytometry and image analysis capabilities. The FC-SR also operates a FACS Aria III Sorter that serves as the primary workhorse for analysis and cell sorting.  Currently, the facilty also operates a FC500 Cell Analyzer for routine cell analyses. The data analysis capabilities of the facility have advanced greatly with the addition of many FlowJo site licenses and the development of new advanced data analysis capabilities.

 
Service Non-PCCR PCCR
iCyt Reflection Cell Sorter $ 45.00 $ 45.00
Beckman Quantum Analyzer $ 28.00 $ 12.00
Beckman FC500 Analyzer $ 42.00 $ 35.00
Cyntellect LEAP cell processing $ 45.00 $ 31.00
FACSAria III Cell Sorter $ 45.00 $ 30.00
Hourly Staff Rate $ 57.00 $ 38.00
 

Location of Flow Cytometry SR Facility